What is a fluorescence polarization assay?
Fluorescence polarization (FP) technology is based on the measurement of molecule rotation, and has been widely used to study molecular interactions in solution. This method can be used to measure binding and dissociation between two molecules if one of the binding molecules is relatively small and fluorescent.
How do fluorescent immunoassays work?
Fluorescent Immunoassays are simply a different type of immunoassay. A modern fluorescent based immunoassay uses as the detection reagent a fluorescent compound which absorbs light or energy (excitation energy) at a specific wavelength and then emits light or energy at a different wavelength.
What is fluorescence assay?
Fluorescence assays are the most common optical detection techniques; they are extensively used in the design of bioassays and biosensors due to their high sensitivity, high efficiency, and simple operation [39].
Why is fluorescence polarized?
Fluorescence polarization (FP) is a fluorescence-based detection method that is widely used to monitor molecular interactions in solution. FP is typically used to assess biomolecular interactions such as protein protein and protein DNA binding, as well as enzyme activity.
What do immunoassays do?
Immunoassay tests work by using antibodies to detect small biological substances in the blood and other bodily fluids. The method follows the aspect that particular antigens binds to specific newly introduced antibodies, thereby stimulating an immune response.
What is the principle of immunoassay?
The principle behind the Immunoassay test is the use of an antibody that will specifically bind to the antigen of interest. The antibodies used in the Immunoassay must have a high affinity for the antigen. The antibodies used in the Immunoassay can either be monoclonal or polyclonal antibodies.
What is fluorescence polarization in immunoassay?
S.N. Hajare, in Encyclopedia of Food Microbiology (Second Edition), 2014 Fluorescence polarization (FP) immunoassay is based on the competition between mycotoxin and a mycotoxin–fluorescein tracer for a mycotoxin-specific antibody.
What is the principle of fluorescence polarization analyzer?
Principle. The solution is evaluated again by the fluorescence polarization analyzer. The fluorescence polarization value for the tracer containing solution is compared to the baseline and magnitude of difference is proportional to quantity of target analyte in sample.
What happens when fluorophores are exposed to plane polarized light?
When a fluorophore in solution is exposed to plane-polarized light at its excitation wavelength, the resulting emission is depolarized. This depolarization results from the motion of the fluorophore during the process of excitation and emission.
What is the fluorescence anisotropy assay?
The basis of the assay is fluorescence anisotropy, also known as fluorescence polarization. If a fluorescent molecule is stationary and exposed to plane-polarized light, it will become excited and consequently emit radiation back to the polarized-plane.