What are HepG2 cells?
HepG2 is a human hepatoma that is most commonly used in drug metabolism and hepatotoxicity studies. HepG2 cells are nontumorigenic cells with high proliferation rates and an epithelial-like morphology that perform many differentiated hepatic functions.
What is smc7721?
The SMMC-7721 cell line was derived from an Asian male patient with hepatocellular carcinoma in 1977. Human hepatoma cell line SMMC-7721 expresses EGFR and VEGFR and is an invaluable tool in liver cancer research.
What is the shape of liver cell?
The cells are polygonal in shape and their sides can be in contact either with sinusoids (sinusoidal face) or neighboring hepatocytes (lateral faces). A portion of the lateral faces of hepatocytes is modified to form bile canaliculi.
Are HepG2 cells hepatocytes?
Hep G2 (or HepG2) is a human liver cancer cell line. Hep G2 will respond to stimulation with human growth hormone. Hep G2 cells are a suitable in vitro model system for the study of polarized human hepatocytes. Another well-characterized polarized hepatocyte cell line is the rat hepatoma-derived hybrid cell line WIF-B.
What does HepG2 stand for?
HepG2 (Liver Hepatocellular Carcinoma) Cell Line.
What do the hepatocytes do?
Hepatocytes, the major parenchymal cells in the liver, play pivotal roles in metabolism, detoxification, and protein synthesis.
Are hepatocytes terminally differentiated?
Terminally Differentiated Cells For example, during liver damage, hepatocytes proliferate. Terminally differentiated cells are another important target for small molecules in in vivo tissue regeneration.
What are primary human hepatocytes?
Hepatocytes directly isolated from liver tissue are called primary hepatocytes. Many other cell culture models have been used to simulate the liver environment for research and development. Some of these alternative hepatocyte models include tumor cell lines such as Huh-7 and HepG2 cells.
What are hep2 cells?
The HEp-2 cell, a native protein array with hundreds of antigens, provides an ideal substrate for the detection of ANA1. The detection of ANA in human serum is an important screening tool for connective tissue diseases, and IIF is the reference method for ANA testing1.
What kind of cells are hepatocytes?
The hepatocytes (epithelial cells of the liver) form branching plates of cells, often only one cell thick, between a system of capillary sinusoids that connect the portal tracts to the central vein.
Do hepatocytes produce digestive enzymes?
If secreted in their active forms, they would self-digest the pancreas. These enzymes are activated in the duodenum. The hepatocytes are the main cell type of the liver. They process, store, and release nutrients into the blood.
Are L02(a) and HepG2(B) cells apoptotic?
Figure Legend Snippet: L02 (a) and HepG2 (b) cells were cultured in 6-well plates for 48 hours, then apoptosis of L02 cells and HepG2 cells was detected by flow cytometry. TiO 2 increased the percentage of apoptotic liver cancer cells. Annexin V-positive cells were considered as apoptotic liver cancer cells.
Can human hepatocyte line l-02 improve liver function and survival?
L-02 cells, expressing the main molecules of human hepatocytes, improved liver functions and survival of ALF rats, suggesting them to be a feasible source for liver support systems. Human fetal hepatocyte line, L-02, exhibits good liver function in vitro and in an acute liver failure model
Does l-02 show liver function markers in human liver disease?
Results: In vitro, Western blotting and laser confocal microscopy showed L-02 to express liver function markers: Albumin (ALB), uridine diphosphate glucuronosyltransferase, and cytochrome P450 3A4. PCR showed the expression of liver-specific genes, such as ALB, human glutathione S transferase, and human factor-X.
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